Apparatus and methods for magnetic separation

Details Apparatus and methods for magnetic separation Apparatus and methods for magnetic separation

B - Operations, Transporting

01

D

B01D 35/06 (2006.01) B03C 1/01 (2006.01) B03C 1/032 (2006.01) B03C 1/033 (2006.01) B03C 1/034 (2006.01) C12M 3/00 (2006.01) C12Q 1/00 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 35/00 (2006.01) G01N 35/02 (2006.01)

Patent

CA 2087037

Magnetic separation ap- paratus and method for sepa- rating colloidal magnetic parti- cles from a non-magnetic test medium in which the magnetic particles are suspended. The separator comprises a contain- er (23) holding the non-magne- tic test medium, one or more magnetic wires (25) disposed substantially within the test medium in the container and an external magnet (31) for producing a magnetic field gra- dient within the test medium. According to the method of the invention, the container (23) holding; the test medium is po- sitioned in the separator (21), producing a magnetic field gra- dient operative to cause the magnetic particles to be attract- ed to the areas surrounding the magnetized wires (25) and to adhere to the wires. The non-magnetic test medium is separated from the magnetized particles, which may conveniently be washed while adhered to the wires, and subjected to further analysis, prefetably while on the wires. The apparatus and method are useful in separating various target substances from test media by means of substances coated on the magnetic particles which bind specifically to the target substance. A modified buffer solution, when added to the test medium, reduces non-specific binding of the magnetic colloid particles to cells.

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