Eia for monitoring legionella pneumophila presence in water...

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G01N 33/18 (2006.01) G01N 31/22 (2006.01) C12Q 1/06 (2006.01) G01N 1/40 (2006.01)

Patent

CA 2393628

A qualitative and quantitative EIA for detecting L. pneumophila in water samples is disclosed. Critical to the disclosed levels of sensitivity of these EIA's is the use of antigen-specific antibodies to the target L. pneumophila antigen that have been rendered antigen-specific by affinity purification on a chromatographic column, which antibodies and their purification are described in detail in parent application Serial No. 09/139,720 filed August 25, 1998.

L'invention concerne un EIA quantitatif et qualitatif destiné à détecter L. pneumophila dans des échantillons d'eau. L'utilisation d'anticorps spécifiques de l'antigène cible L. pneumophila rendus spécifiques de l'antigène par purification par affinité sur une colonne chromatographique est un élément crucial pour déterminer les niveaux de sensibilité de ces EIA. Ces anticorps et leur purification sont décrits en détail dans la demande principale numéro de série 09/139,720 déposée le 25 août 1998.

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