Synthesis and purification of hepatitis c virus-like particles

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/40 (2006.01) A61K 39/29 (2006.01) C07K 14/18 (2006.01) C07K 16/10 (2006.01) C12N 5/10 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2269097

Production of enveloped RNA virus-like particles intracellularly in vitro in insect cells using a recombinant baculovirus vector containing a cDNA coding for viral structural proteins is disclosed. In vitro production and purification of hepatitis C virus (HCV)-like particles containing HCV core protein, E1 protein and E2 protein is disclosed. Production of antibodies in vivo to the purified HCV-like particles is disclosed.

Production intracellulaire in vitro dans des cellules d'insectes de particules de type virus à ARN enveloppé, au moyen d'un vecteur baculovirus recombiné contenant un codage ADNc pour des protéines structurelles virales. L'invention porte en outre sur la production in vitro et la purification de particules de type virus de l'hépatite C (VHC) contenant la protéine noyau VHC, la protéine E1 et la protéine E2, et sur la production in vivo d'anticorps contre les particules de type VHC purifiées.

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