C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/00 (2006.01) C07K 1/30 (2006.01) C12N 1/06 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2710777
This invention relates to a method for lysing cells. The method comprises simultaneously flowing a cell suspension and a lysis solution through a static mixer, wherein the cells exit the static mixer, wherein the cells exit the static mixer lysed. In another aspect of the present invention, the invention relates to a method for precipitating cell components, protein and nucleic acids from a cell lysate or other solution containing precipitable material. The method comprises simultaneously flowing a cell lysate or other protein containing solution and precipitating solution through a static mixer, wherein the lysate or protein solution exits the static mixer with its precipitable components precipitated. In another aspect of the present invention, the invention relates to a method where the two above-mentioned methods are combined by using static mixers in series.
Christopher Charles William
Mcneilly David S.
Wan Nick C.
Genzyme Corporation
Gowling Lafleur Henderson Llp
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