Methods and compositions with reduced opalescence

Details Methods and compositions with reduced opalescence Methods and compositions with reduced opalescence

A - Human Necessities

61

K

A61K 39/395 (2006.01) A61K 9/00 (2006.01) A61K 47/00 (2006.01)

Patent

CA 2665423

Methods for reducing the opalescent appearance of a protein preparation by modifying the ionic strength of the preparation, as well as compositions, e.g., pharmaceutical compositions, of concentrated protein with decreased opalescence are disclosed. Purification methods which monitor and/or reduce the salt concentrations at selected steps are also disclosed.

L'invention concerne des procédés pour réduire l'aspect opalescent d'une préparation de protéines en modifiant la force ionique de la préparation, ainsi que les compositions, par exemple des compositions pharmaceutiques, composées de protéines concentrées ayant une opalescence diminuée sont décrites. Des procédés de purification qui surveillent et/ou réduisent les concentrations en sel à des étapes sélectionnées sont également décrites.

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