Immunoassay for pyridostigmine

Details Immunoassay for pyridostigmine Immunoassay for pyridostigmine

G - Physics

01

N

G01N 33/534 (2006.01) G01N 33/53 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2051618

A process for determining the concentration of pyridostigmine in a sample of biological fluid, comprises mixing the sample with antibody produced from antigen formed from the synthesis of pyridostigmine-p-aminobenzoic acid-bovine serum albumin complex, determining the extent of binding reaction between antibody and pyridostigmine and comparing the measured extent of binding an unknown with known quantitative relationship between binding and concentration of pyridostigmine.

On a mis au point un procédé de détermination de la concentration de pyridostigmine dans un échantillon de fluide biologique, consistant à mélanger l'échantillon avec un anticorps obtenu à partir d'un antigène formé par synthèse d'un complexe d'albumine de sérum bovin et d'acide pyridostigmine-p-aminobenzoïque, à déterminer l'ampleur de la réaction de liaison entre ledit anticorps et la pyridostigmine, et à comparer le degré mesuré de liaison d'une inconnue à un rapport quantitatif connu entre la liaison et la concentration de la pyrridostigmine.

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