Fluorescent proteins from aquatic species

Details Fluorescent proteins from aquatic species Fluorescent proteins from aquatic species

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) A01K 67/027 (2006.01) C07K 14/435 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2509373

Provided are four new fluorescent proteins. The proteins were derived from two wild-type fluorescent proteins: a red fluorescent protein (RFP) that was isolated from Actinodiscus or Discosoma sp. 1 and a green fluorescent protein (GFP) isolated from Montastraea cavernosa. Two mutant forms were generated from each wild-type protein. Each of the mutated forms has a higher fluorescence intensity than the respective wild-type form. The mutant forms of the fluorescent proteins allow for more sensitive detection of the fluorescence emitted by the proteins. Additionally, one of the mutant proteins is more resistant to photobleaching than its wild-type protein. The invention also encompasses isolated nucleic acids encoding the mutant forms of the wild- type RFP and GFP.

L'invention concerne quatre nouvelles protéines fluorescentes. Les protéines ont été dérivées de deux protéines fluorescentes de type sauvage: une protéine fluorescente rouge (RFP) isolée de <i>Actinodiscus</i> ou <i>Discosoma sp. 1</i> et une protéine fluorescente verte (GFP) isolée de <i>Montastraea cavernosa</i>. Deux formes mutantes ont été produites à partir de chaque protéine de type sauvage. Chaque forme mutée présente une intensité de fluorescence plus élevée que la forme de type sauvage respective. Les formes mutantes des protéines fluorescentes permettent d'obtenir une détection plus sensible de la fluorescence émise par les protéines. De plus, une des protéines mutantes est plus résistante à la photo-instabilité que la protéine de type sauvage respective. L'invention concerne également des acides nucléiques isolés codant les formes mutantes des RFP et GFP de type sauvage.

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