C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/26 (2006.01) C12Q 1/02 (2006.01)
Patent
CA 2489935
A method for measuring polyamines in erythrocytes in which a polyamine oxidase having a substrate specificity to spermine and spermidine or a polyamine oxidase having a substrate specificity to spermine only is used, an eluate in erythrocytes separated and purified from blood is reacted with these enzymes, and hydrogen peroxide formed is determined with a highly sensitive chromogen such as a diphenylamine-based chromogen. There is provided a method for measuring amounts of polyamines in erythrocytes easily with high precision. The method is effective for diagnosis of certain disease conditions or physiological conditions or the like of subjects.
National University Corporation Nagoya University
Smart & Biggar
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