Long lifetime anisotropy (polarization) probes for clinical...

G - Physics – 01 – N

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G01N 33/542 (2006.01) G01N 21/21 (2006.01) G01N 33/533 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2203772

A method of conducting an immunoassay of a sample of interest is described, including the steps of: (A) coupling a luminescent asymmetric metal-ligand complex to the sample of interest to form a coupled sample; (B) exciting the coupled sample with linearly polarized electromagnetic energy to cause the coupled sample to emit fluorescent light; and (C) measuring the polarization of the fluorescent emission as a measure of a biological characteristic of the sample of interest. The measurement technique is schematically illustrated in the figure.

Procédé d'exécution d'un essai immunologique sur un échantillon d'intérêt consistant: A) à coupler un complexe lumineux asymétrique métal/ligand sur l'échantillon pour former un échantillon couplé, B) à exciter l'échantillon couplé par de l'énergie électromagnétique polarisée pour amener l'échantillon couplé à émettre de la lumière fluorescente, et C) à mesurer la polarisation de l'émission fluorescente qui constitue une mesure d'une des caractéristiques biologiques de l'échantillon. La technique de mesure est illustrée dans la figure.

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