Methods

Details Methods Methods

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) C12N 1/21 (2006.01) C12N 5/00 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2358263

The present invention relates to a method for the production of a minicircle. In the method of the present invention, a parent plasmid is provided which has a nucleic sequence flanked by recombination sites. This parent plasmid is exposed to an enzyme which causes recombination at the recombination sites, thereby to form a (i) minicircle comprising the nucleic acid sequence and (ii) a miniplasmid comprising the remainder of the parent plasmid. One recombination site is modified at the 5' end such that its reaction with the enzyme is less efficient than the wild type site, and the other recombination site is modified at the 3' end such that its reaction with the enzyme is less efficient than the wild type site, both modified sites being located in the minicircle after recombination. This favours the formation of minicircle.

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