Plant virus-based inducible expression system

Details Plant virus-based inducible expression system Plant virus-based inducible expression system

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01)

Patent

CA 2648886

A process of producing one or more than one protein of interest, comprising: (a) providing a plant or plant cells comprising a first heterologous nucleotide sequence comprising a nucleotide sequence encoding an RNA replicon, and a first inducible promoter operably linked to said nucleotide sequence encoding said RNA replicon; said RNA replicon not encoding a protein providing for cell-to-cell movement of said RNA replicon in said plant; said RNA replicon encoding a polymerase and said one or more than one protein of interest, said polymerase being adapted for replicating said RNA replicon; and (b) inducing, in said plant or plant cells of step (a), said inducible promoter, thereby producing said one or more than one protein of interest in said plant or plant cells.

Un processus de production d'une ou plusieurs protéines d'intérêt, consistant à : (a) fournir une plante ou des cellules végétales comprenant une première séquence hétérologue de nucléotides comprenant une séquence de nucléotides codant un ARN réplicon, et un premier promoteur inductible lié de façon opérationnelle à ladite séquence de nucléotides codant ledit ARN réplicon ; ledit ARN réplicon ne codant pas une protéine qui fournit un déplacement de cellule à cellule dudit ARN réplicon dans ladite plante ; ledit ARN réplicon codant une polymérase et ladite ou lesdites protéines d'intérêt, ladite polymérase étant adaptée pour répliquer ledit ARN réplicon ; et (b) induire, dans ladite plante ou lesdites cellules végétales de l'étape (a), ledit promoteur inductible et, de ce fait, produire ladite ou lesdites protéines d'intérêt dans ladite plante ou lesdites cellules végétales.

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