Method of isolation primer extension products with modular...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2343072

The invention provides a method of isolating primer extension products, particularly sequencing reaction products, in which the products contain sequences corresponding or complementary to i) a primer binding region, ii) an insert and iii) vector-derived sequence(s), and a modular oligonucleotide of at least two parts which is complementary to the vector-derived sequence(s) and binds to adjacent stretches of the primer extension products is used for isolation, the modular oligonucleotides themselves and their use in methods of the invention.

L'invention concerne un procédé d'isolation de produits d'extension d'amorces, notamment de produits de réaction de séquençage, lesquels produits contiennent des séquences correspondant à ou complémentaire de i) une région de fixation d'amorce, ii) un segment d'insertion et iii) une (des) séquence(s) dérivées de vecteurs ; elle concerne également l'utilisation, pour cette isolation, d'un oligonucléotide modulaire, lequel comprend au moins deux parties, est complémentaire de la (des) séquence(s) dérivées de vecteurs et se fixe sur des fragments adjacents des produits d'extension d'amorces. L'invention concerne également ces oligonucléotides ainsi que l'utilisation de ceux-ci dans des procédés de l'invention.

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