Light emission modifiers and their uses in nucleic acid...

Details Light emission modifiers and their uses in nucleic acid... Light emission modifiers and their uses in nucleic acid...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12M 1/34 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2549671

The present invention relates to methods and reagents for modifying the emission of light from labeled nucleic acids for the purpose of real time detection, analysis, and quantitation of nucleic acid sequences, e.g., using singly labeled probes. These methods and reagents exploit advantageous properties of thiazine dyes and diazine dyes. Furthermore, the use of these light emission modifiers in background reduction, nucleic acid duplex stabilization and other uses is also described. Related kits, reaction mixtures and integrated systems are described.

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