System and method for the production of recombinant...

Details System and method for the production of recombinant... System and method for the production of recombinant...

C - Chemistry, Metallurgy

12

N

C12N 9/10 (2006.01) C12N 15/70 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2477794

A system and a method for the production of recombinant N-glycosylated target proteins. The system comprises a prokaryotic organism (e.g. Escherichia coli) into which is introduced a genetic information encoding for a metabolic apparatus capable of carrying out the requested N-glycosylation of the target protein. Said prokaryotic organism also contains the genetic information required for the expression of one or more recombinant target proteins. The metabolic apparatus preferably comprises specific glycosyltransferases for the assembly of the oligosaccharide on a lipid carrier and an OTase that covalently links this oligosaccharide to specific residues of the desired protein.

L'invention concerne un système et un procédé de fabrication de protéines cibles N-glycosylées. Le système selon l'invention comporte un organisme procaryotique (par ex. <i>Escherichia coli) dans lequel une information génétique est intégrée, ladite information codant un appareil métabolique pouvant effectuer la N-glycosylation requise de la protéine cible. Ledit organisme procaryotique contient également l'information génétique nécessaire à l'expression d'une ou plusieurs protéines cibles de recombinaison. Ledit appareil métabolique comporte de préférence des glycosyltransférases spécifiques destinées à l'assemblage des oligosaccharides sur un support lipidique, ainsi qu'une OTase liant par covalence les oligosaccharides à des résidus spécifiques de la protéines d'intérêt.

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