Methods of amplifying and sequencing nucleic acids

Details Methods of amplifying and sequencing nucleic acids Methods of amplifying and sequencing nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) C40B 20/00 (2006.01) C40B 50/06 (2006.01) C40B 50/14 (2006.01) G01N 21/64 (2006.01) C40B 40/06 (2006.01)

Patent

CA 2513899

An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

La présente invention a trait à un appareil et un procédé pour la réalisation rapide de séquençage de l'ADN, tel que le séquençage génomique. Le procédé comprend les étapes suivantes : la préparation d'un échantillon d'ADN pour le séquençage génomique, l'amplification de l'ADN préparé d'une manière représentative, et la réalisation d'une réaction de séquençage multiple sur l'ADN amplifié avec seulement une étape d'hybridation d'amorce.

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