Methods for preparing immunogenic compositions

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/00 (2006.01) A61K 39/145 (2006.01) A61P 31/00 (2006.01) A61P 31/16 (2006.01) A61P 37/04 (2006.01) C07K 1/14 (2006.01) C07K 14/195 (2006.01) C07K 14/22 (2006.01) C11B 1/10 (2006.01)

Patent

CA 2722558

The present invention relates methods of preparing a composition comprising the steps of: releasing from at least one cell a composition comprising at least one outer membrane protein from said at least one cell comprising contacting said at least one said cell with at least one agent capable of solubilizing at least one lipid and optionally an osmalalic agent, forming a mixture comprising said at least one outer membrane protein, at least one endogenous liposaccharide and cell debris; adding an agent capable of separating said cell debris from said at least one outer membrane protein; separating said separated cell debris from said mixture; removing at least one agent capable of solubilizing at least one lipid and said optional osmolalic agent from said at least one outer membrane protein wherein said at least one outer membrane protein remains soluble. Also, included within this invention are compositions comprising at least one outer membrane protein and an endogenous liposacharide wherein said en-dogenous liposaccharide is in the range of about 0.03 grams to about 0.99 grams endogenous liposaccharide to 1.0 gram of at least one outer membrane protein weight.

La présente invention concerne des procédés de préparation dune composition comprenant les étapes consistant à : libérer dau moins une cellule une composition comprenant au moins une protéine de membrane externe provenant de ladite au moins une cellule comprenant la mise en contact de ladite au moins une cellule avec au moins un agent qui peut solubiliser au moins un lipide et éventuellement un agent dosmolalité, formant un mélange comprenant ladite au moins une protéine de membrane externe, au moins un liposaccharide endogène et des débris cellulaires; ajouter un agent qui peut séparer lesdits débris cellulaires de ladite au moins une protéine de membrane externe; séparer lesdits débris cellulaires séparés dudit mélange; éliminer au moins un agent qui peut solubiliser au moins un lipide et ledit agent dosmolalité éventuel de ladite au moins une protéine de membrane externe où ladite au moins une protéine de membrane externe reste soluble. De plus, linvention concerne des compositions comprenant au moins une protéine de membrane externe et un liposaccharide endogène où ledit liposaccharide endogène est dans la gamme denviron 0,03 gramme à environ 0,99 gramme de liposaccharide endogène à 1,0 gramme dau moins une protéine de membrane externe en poids.

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