Method and devices for forming a plurality of wells on a gel

G - Physics – 01 – N

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G01N 27/447 (2006.01) C07K 1/14 (2006.01)

Patent

CA 2612052

The present invention relates to methods and devices for forming a plurality of wells on a gel containing an analyte. The invention further provides a system for eluting a liquid analyte reagent mixture from a gel. The invention is useful in the separation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for separating and eluting peptides from isoelectric focusing gels.

La présente invention a pour objet des méthodes et dispositifs pour former une multitude de puits sur un gel qui contient un analyte. L'invention fournit en outre un système pour éluer un mélange du réactif liquide d'analyte à partir d'un gel. L'invention est utile pour la séparation de molécules biologiques tel que des acides nucléiques, hydrates de carbone, protéines et peptides. En particulier, l'invention est utile pour séparer et éluer des peptides à partir de gels pour isoélectrofocalisation.

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