Protein assay method and kit

Details Protein assay method and kit Protein assay method and kit

G - Physics

01

N

G01N 33/68 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2083287

A method for protein assay is a modified Lowry method in which a Step 1 reaction between a copper-containing alkaline solution and a protein solution takes place at high alkali concentration. The Step 2 reaction between the products of Step 1 and Folin reagent commences at a pH of between 11 and 12 in order to produce rapidly the coloured species on the basis of which a maximum optical density is reached in a relatively short time and remains constant for a sufficient period to enable the required number of optical density determinations to be made.

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