Exonuclease decontamination method

Details Exonuclease decontamination method Exonuclease decontamination method

C - Chemistry, Metallurgy

12

S

C12S 3/20 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2101119

EXONUCLEASE DECONTAMINATION METHOD Abstract An amplicon decontamination method employing single strand-specific exonucleases is disclosed. The method comprises combining a nucleic acid preparation containing target nucleic acid, which preparation is potentially contaminated with nucleic acid amplification reaction products (amplicons), with a single-strand specific exonuclease, and incubating the preparation with the exonuclease for a time sufficient to degrade the amplicons. The amplicons may be the products of a strand displacement amplification reaction (SDA amplicons). Suitable exonucleases include exonuclease I, exonuclease VII, and DNA polymerases possessing 3'-5' exonuclease activity.

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