Methods for forming dyed microspheres and populations of...

C - Chemistry – Metallurgy – 09 – B

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C09B 69/10 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2583531

Various methods for forming dyed microspheres are provided. One method includes activating a chemical structure coupled to a dye using heat or light to form a reaction intermediate in the presence of a microsphere. The reaction intermediate covalently attaches to a polymer of the microsphere thereby coupling the dye to the polymer and forming the dyed microsphere. Additional methods are provided for forming a dyed microsphere coupled to a molecule. These methods include dyeing the microspheres as described above in addition to synthesizing the molecule on an outer surface of the dyed microspheres. A population of dyed microspheres is also provided. Each of the dyed microspheres of the population includes a dye attached to a polymer of each of the dyed microspheres by a chemical structure. A coefficient of variation in dye characteristics of the population of dyed microspheres attributable to the dye is less than about 10%.

L'invention concerne divers procédés de formation de microsphères colorées. Un procédé consiste à activer une structure chimique couplée à un colorant qui utilise la chaleur ou la lumière pour produire une réaction avec un intermédiaire en présence d'une microsphère. L'intermédiaire de la réaction s'attache par covalence à un polymère de la microsphère, de manière à coupler le colorant au polymère et à former la microsphère colorée. Des procédés supplémentaires sont prévus pour former une microsphère colorée, couplée à une molécule. Ces procédés consistent à colorer les microsphères décrites ici et, de plus, synthétiser une molécule sur une surface externe des microsphères colorées. L'invention porte aussi sur une population de microsphères colorées. Chacune des microsphères colorées comprend un colorant attaché à un polymère de chacune des microsphères colorées au moyen d'une structure chimique. Un coefficient de variation des caractéristiques de la population de microsphères colorées, qui peut être attribué au colorant, est inférieur à environ 10 %.

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