Method and device for detecting feline immunodeficiency virus

Details Method and device for detecting feline immunodeficiency virus Method and device for detecting feline immunodeficiency virus

C - Chemistry, Metallurgy

07

K

C07K 16/10 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2570610

A method and device for determining a feline immunodeficiency virus infection or vaccination in an animal. The method is an assay involving contacting a biological sample from a felid with anenv FIV polypeptide and determining the binding of antibodies in the sample to the polypeptide. The assay is optimized by adjusting the concentration of the sample and reagents, and the time and temperature of the incubation, so that antibodies in samples from vaccinated animals do not substantially bind to the polypeptide.

Un procédé et un dispositif pour déterminer une infection par virus d'immunodéficience féline ou la vaccination chez un animal. Le procédé est un test impliquant le contact entre un échantillon biologique d'un félidé avec un polypeptide FIV env et déterminant la liaison des anticorps de l'échantillon au polypeptide. Le test est optimisé si l'on ajuste la concentration de l'échantillon et des réactifs et le délai et la température de l'incubation de sorte que les anticorps de l'échantillon des animaux vaccinés ne se lient pas sensiblement au polypeptide.

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