Recombinant vaccine for diseases caused by encapsulated...

A - Human Necessities – 61 – K

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A61K 39/00 (2006.01) A61K 39/02 (2006.01) A61K 39/102 (2006.01) C07K 14/285 (2006.01) C12N 1/36 (2006.01)

Patent

CA 2258168

Vaccines for diseases caused by normally encapsulated organisms are produced by genetically modifying those organisms by deleting the genes encoding for capsule synthesis or a portion thereof sufficient to produce non-capsulated mutants of the organisms. As an example, a live, attenuated strain of Actinobacillus pleuropneumoniae genetically modified with a large deletion in chromosomal regions of the DNA which encodes for capsule synthesis is a safe and effective vaccine against swine pleuropneumonia.

La présente invention concerne des vaccins destinés à des pathologies habituellement imputables à des organismes encapsulés. Ces vaccins sont produits en modifiant génétiquement de tels organismes. Cette modification consiste, à leur supprimer les gènes codant pour la synthèse de capsule, ou à leur supprimer une partie suffisamment importante de ces gènes, pour arriver à la production de mutants non encapsulés de ces organismes. C'est ainsi, par exemple, qu'une souche vivante atténuée d'Actinobacillus pleuropneumoniae, génétiquement modifiée par une importante suppression dans des régions chromosomiques de l'ADN codant pour la synthèse de la capsule, constitue un vaccin sain et efficace contre la pleuro-pneumonie porcine.

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