Method for isolating hepatocytes

G - Physics – 01 – N

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Details

G01N 33/483 (2006.01) G01N 33/50 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2539002

The present invention provides a method for isolating normal hepatocytes, the method comprising the steps of: recovering liver tissue from a patient during a hepatectomy; and isolating normal hepatocytes from unwanted cells present in the recovered tissue by magnetic separation. The invention further provides a method of preparing hepatocytes for transplantation, the method comprising the steps of: recovering liver tissue from a patient during a hepatectomy; and isolating normal hepatocytes from unwanted cells present in the recovered tissue by magnetic separation.

L'invention concerne un procédé d'isolation d'hépatocytes normales, qui consiste à: prélever un tissu hépatique chez un patient par hépatectomie; et isoler des hépatocytes normales à partir des cellules non désirées présentes dans le tissu prélevé par séparation magnétique. Par ailleurs, l'invention concerne un procédé de préparation d'hépatocytes en vue d'une transplantation, qui consiste à: prélever un tissu hépatique chez un patient par hépatectomie; et isoler des hépatocytes normales à partir des cellules non désirées présentes dans un tissu prélevé par séparation magnétique.

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