Isolation of microbial oils

C - Chemistry – Metallurgy – 11 – C

Patent

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Details

C11C 1/10 (2006.01) A23D 9/00 (2006.01) C11B 1/00 (2006.01) C11B 1/02 (2006.01) C12N 1/06 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2417571

The extraction of a microbial or single cell oil, for example comprising one or more polyunsaturated fatty acids (PUFAs), directly from microbial cells is disclosed which avoids the need for solvents. After fermentation, the microbial cells are pasteurised, washed and the cell walls lysed or disrupted by a mechanical (e.g. homogenisation), physical (boiling or drying), chemical (solvents) or enzymatic (cell wall degrading enzymes) technique. The oil (containing the PUFA) is then separated from the resulting cell wall debris. This is achieved by centrifugation, which results is an oily phase (top layer) that contains the oil which that can be separated from an aqueous phase (containing the cell wall debris). The oil can then be extracted and if necessary the PUFA can be purified or isolated from the oil.

L'invention concerne l'extraction d'une huile microbienne ou unicellulaire, contenant par exemple un ou plusieurs acides gras polyinsaturés, directement de cellules microbiennes évitant l'utilisation de solvants. Après fermentation, les cellules microbiennes sont pasteurisées, lavées et les parois cellulaires sont lysées ou dissociées par une technique mécanique (par exemple homogénéisation), physique (ébullition ou séchage), chimique (solvants) ou enzymatique (enzymes dégradant la paroi cellulaire). L'huile (contenant les acides gras polyinsaturés) est ensuite séparée des débris de paroi cellulaire résultants. Ceci est obtenu par centrifugation, produisant une phase huileuse (couche supérieure) contenant l'huile pouvant être séparée d'une phase aqueuse (contenant les débris de paroi cellulaire). L'huile peut ensuite être extraite et si nécessaire l'acide gras polyinsaturé peut être purifié ou isolé de l'huile.

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