Production of glycoproteins

Details Production of glycoproteins Production of glycoproteins

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01)

Patent

CA 2657248

An improved system for large scale production of glycoproteins in cell culture is provided. In accordance with the present invention, cells expressing a glycoprotein are grown in media that contain manganese at a concentration of between approximately 10 and 600 nM. The use of such a system allows production of a glycoprotein with an increased glycosylation pattern and/or a glycosylation pattern that more accurately reflects the glycosylation pattern of the naturally occurring glycoprotein. A glycoprotein expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical compositions.

L'invention concerne un système amélioré pour produire des glycoprotéines à grande échelle dans une culture cellulaire. Selon la présente invention, des cellules exprimant une glycoprotéine sont cultivées dans des milieux contenant du manganèse à une concentration allant approximativement de 10 à 600 nM. L'utilisation d'un tel système permet la production d'une glycoprotéine dotée d'un motif de glycosylation augmenté et/ou d'un motif de glycosylation qui reflète plus précisément le motif de glycosylation de la glycoprotéine apparaissant dans la nature. Une glycoprotéine exprimée selon la présente invention peut être avantageusement utilisée dans la préparation de compositions pharmaceutiques.

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