Epitopic regions of pneumococcal surface protein a

Details Epitopic regions of pneumococcal surface protein a Epitopic regions of pneumococcal surface protein a

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) A61K 39/09 (2006.01) C07H 21/04 (2006.01) C07K 14/315 (2006.01) C07K 14/715 (2006.01) C12Q 1/68 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2149345

Regions of the pneumococcal surface protein A (PspA) protein of the Rx1 strain of Streptococcus pneumoniae have been identified as containing protection-eliciting epitopes. One region comprises the 68-amino acid sequence extending from amino acid residues 192 to 260 of the Rx1 PspA strain, another region comprises the C- terminal amino acid sequence extending from amino acid residues 192 to 588 and a third region comprises the C- terminal amino acid sequence extending from amino acid residues 293 to 588 of the Rx1 PspA strain. Individual oligonucleotide primers and probes and described which may be used in specific pairs to amplify pneumococcal DNA from a variety of strains of S. pneumoniae for the detection or amplification of such DNA. Full-length PspA is also capable of providing cross-protection against a variety of pneumococcal strains.

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