Arrangement for the optical excitation of fluorescent...

Details Arrangement for the optical excitation of fluorescent... Arrangement for the optical excitation of fluorescent...

G - Physics

01

N

G01N 21/64 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2387614

The invention is directed to an arrangement for reading out the fluorescent radiation of specimen carriers with a plurality of individual specimens which for purposes of exciting fluorescent radiation in selected individual specimens comprises a switchable electro-optical matrix for generating illumination which is limited in a spatially defined manner. In an arrangement for reading out the fluorescent radiation of selected individual specimens of multispecimen carriers (5) having a switchable electro-optical matrix (2) for generating illumination which is limited in a spatially defined manner, an optical system (3) for imaging the electro- optical matrix (2) on the specimen carrier (5), and a high-sensitivity photoreceiver (7) for integral measurement of the fluorescent radiation of the excited individual specimens of the specimen carrier (5), the object of the invention, to find a novel possibility for a spatially differentiated illumination of a specimen carrier (5) with a plurality of specimens using an electro-optical matrix (2) which minimizes the proportion of excitation radiation contributing to the fluorescence signal in high- resolution imaging of the electro-optical matrix (2) and the specimen carrier (5) are inclined relative to the optical axis (31) of the optical system (3) and are subject to a Scheimpflug condition, and the angles of inclination (.alpha.; .beta.) of the electro-optical matrix (2) and of the specimen carrier (5) are selected such that the excitation radiation imaged by the light source unit (1) on the specimen carrier (5) is reflected in such a way that essentially no excitation radiation reaches the detection beam path (53).

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