Density gradient medium for separating cells

Details Density gradient medium for separating cells Density gradient medium for separating cells

B - Operations, Transporting

01

D

B01D 21/26 (2006.01) C12Q 1/24 (2006.01) G01N 33/49 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2182367

A density gradient medium for the isolation and enrichment of rare cells, including fetal nucleated erythrocytes from a peripheral blood sample is described. The medium comprises a colloidal density gradient medium dispersed in a meltable gel. In one aspect of the invention, the density gradient medium is hypertonic to facilitate separation of maternal red blood cells from fetal blood cells.

Milieu de gradient de densité pour l'isolement et l'enrichissement de cellules rares, y compris d'érythrocytes foetaux nucléés, à partir d'un échantillon de sang périphérique. Ledit milieu comprend un milieu colloïdal de gradient de densité dispersé dans un gel fusible. Selon un aspect de la présente invention, le milieu de gradient de densité est hypertonique pour faciliter la séparation des hématies maternelles des hématies foetales.

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