C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/87 (2006.01) C12N 13/00 (2006.01) C12M 1/42 (2006.01)
Patent
CA 2685409
Electroporation of mammalian cells is performed to high efficiency by use of a buffer solution containing trehalose, sucrose, or both, in addition to an inorganic phosphate buffer, an organic sulfonic acid buffer, a halide salt of an alkali or alkaline earth metal, a chelating agent, an alkyl methyl sulfoxide, and a nucleotide triphosphate, with substantially no sodium. This buffer solution is effective both in the electroporation of cells that are suspended in the buffer solution and in the electroporation of cells that are fixed on a surface.
L'invention concerne l'électroporation de cellules de mammifères, que l'on effectue avec une haute efficacité en utilisant une solution tampon contenant du tréhalose, du saccharose ou les deux, ainsi qu'un tampon de phosphate inorganique, un tampon d'acide sulfonique organique, un sel d'halogénure de métal alcalin ou alcalino-terreux, un agent de chélation, un sulfoxyde d'alkylméthyle et un triphosphate nucléotidique, et ne contenant essentiellement pas de sodium. Cette solution tampon est efficace pour l'électroporation de cellules qui sont en suspension dans la solution tampon et pour l'électroporation de cellules qui sont fixées sur une surface.
Rubio Teresa
Terefe Joseph
Bio-Rad Laboratories Inc.
Fetherstonhaugh & Co.
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