C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2440531
Compositions, systems, kits and methods relating to in vitro synthesis are provided. The system includes one or more extract s having reduced activity of an enzyme that catalyses hydrolysis of high energy phosphate bonds or hydrolysis or formation of phosphodiester bonds, an inhibitor that inhibits hydrolysis of high energy phosphate bonds or hydrolysis or formation of phosphodiester bonds, and/or at least two energy sources. The composition may include a nucleic acid template and one or more products of the nucleic acid template.
L'invention concerne des compositions, des systèmes, des kits et des procédés utilisables pour la synthèse in vitro. On décrit un système qui comprend un ou plusieurs extraits à activité réduite d'une enzyme catalysant l'hydrolyse de liaisons phosphate haute énergie ou bien l'hydrolyse ou la formation de liaisons phosphodiester, un inhibiteur d'hydrolyse de liaisons phosphate haute énergie ou bien d'hydrolyse ou de formation de liaisons phosphodiester, et/ou au moins deux sources d'énergie. La composition considérée peut comprendre une matrice d'acides nucléiques et un ou plusieurs produits issus de cette matrice.
Chatterjee Deb K.
Longo Mary C.
Invitrogen Corporation
Mbm Intellectual Property Law Llp
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