C - Chemistry – Metallurgy – 08 – B
Patent
C - Chemistry, Metallurgy
08
B
C08B 37/08 (2006.01) A01N 25/10 (2006.01) A01N 25/26 (2006.01) A23K 1/00 (2006.01) A23K 1/16 (2006.01) A23L 1/056 (2006.01) A23L 1/22 (2006.01) A61K 9/16 (2006.01) C09B 67/02 (2006.01)
Patent
CA 2255080
The present invention relates to chitin beads having a uniform and fine particle size and comprising microsporidian spores whose principal cell wall substance is chitin; chitosan beads comprising N-deacetylated chitin and having a uniform fine particle size; methods for preparing these beads; carriers comprising these beads; and a method for preparing microsporidian spores. Microsporidian spores are proliferated in insect's bodies or cultured cells to obtain chitin beads having the uniform and fine particle size and whose cell wall substance is mainly composed of chitin. The chitin as the major cell wall substance is subjected to N-deacetylation to give chitosan beads. When the insect is a larva of domesticated silkworm, microsporidian spores are proliferated by orally or percutaneously inoculating the spores into the 2nd instar domesticated silkworm larvae in a concentration of 5 × 10 2 to 5 × 10 8 spores/ml, then the proliferated microsporidian spores are isolated from the bodies of the grown 5th instar domesticated silkworm larvae, followed by purifying the resulting spores to give chitin beads having the uniform and fine particle size and chitosan beads can be obtained from the chitin beads in the same manner used above. These beads are used as carriers for the immobilization or encapsulation of a variety of substances such as an antibiotic and a microorganism.
L'invention porte sur des billes de chitine ayant une granulométrie fine et uniforme et constituées de spores de microsporidies contenant la chitine comme constituant principal des parois cellulaires, ainsi que sur des billes de chitosane de granulométrie fine et uniforme et obtenues par N-désacétylation de la chitine précitée. Les spores de microsporidies prolifèrent chez les insectes ou dans les cellules mises en culture pour produire les billes de chitine précitées. Les billes de chitosane sont obtenues par N-désacétylation de la chitine. Lorsque les insectes sont domestiqués sous forme de larves de vers à soie, 5 x 10<2> à 5 x 10<8>/ml de spores de microsporidies sont inoculés par voie orale ou percutanée dans la larve du ver à soie domestiqué ayant mué dans la seconde phase larvaire et prolifèrent dans celle-ci. Puis les spores ayant proliféré sont retirés de la larve du vers à soie domestiqué dans la cinquième phase larvaire et sont épurés pour produire des billes de chitine de granulométrie petite et uniforme à partir desquelles il est possible d'obtenir des billes de chitosane telles que précitées. Ces billes sont utilisées comme supports pour immobiliser ou encapsuler différentes substances telles que des antibiotiques et des micro-organismes.
Hayasaka Shoji
Shirata Akira
Tsukada Masuhiro
Forestry And Japan As Represented By Director General Of National Institute O
Marks & Clerk
National Institute Of Agrobiological Sciences
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