Methods, immunoassays and devices for detection of...

G - Physics – 01 – N

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G01N 33/571 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2612759

Compositions, methods and devices for the detection of anti-lipoidal antibodies and the diagnosis of disease, for example, syphilis, are described. In particular, a method for immobilizing a lipoidal antigen, comprising cardiolipin, lecithin, and cholesterol, on a solid support (such as a nitrocellulose membrane) is described. The ability to immobilize a lipoidal antigen on a membrane satisfies a long-felt need for membrane-based assay for the detection of anti-lipoidal antibodies. Also described are immunoassay devices for concurrently performing treponemal and non-treponemal tests for syphilis.

Compositions, procédés et dispositifs pour la détection d'anticorps anti-lipoïdaux et le diagnostic de maladies, par exemple la syphilis, et en particulier procédé d'immobilisation d'antigène lipoïdal, comprenant de la cardiolipine, de la lécithine et du cholestérol, sur un support solide (du type membrane nitrocellulosique). La capacité à immobiliser un antigène lipoïdal sur une membrane répond au besoin, existant de longue date, en dosage à base de membrane pour la détection d'anticorps anti-lipoïdaux. Dispositifs d'immunodosage pour la conduite simultanée d'essais de type tréponémal et non tréponémal en liaison avec la syphilis.

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