Assay device and method for performing biological assays

B - Operations – Transporting – 01 – L

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Details

B01L 3/00 (2006.01) B01J 19/00 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2745580

The invention provides a multiplexed assay device comprising a reaction chamber and several sets of encoded mi-crocarriers 2 wherein the reaction chamber is a microchannel 1 and wherein the longitudinal movement of the microcarriers 2 is restricted and wherein the microcarriers 2 have a shape in relation to the geometry of the microchannel 1 such that at least two can stand side by side in the microchannel 1 without touching each other and without touching the perimeter of the microchannel 1 and are preferably observable in the reaction chamber. Moreover, the invention provides a method for performing multiplexed as-say based on microcarriers 2 that improves mass transfer, simplifies the preparation and the execution of the assay and facilitates readout of biological reactions and identity of microcarriers 2.

L'invention concerne un dispositif de dosage multiplexé comportant une chambre de réaction et plusieurs ensembles de micro-supports codés 2, la chambre de réaction étant un micro-canal 1, le mouvement longitudinal des micro-supports 2 étant limité et les micro-supports 2 présentant une forme en rapport avec la géométrie du micro-canal 1 de telle sorte qu'au moins deux d'entre eux puissent se trouver côte à côte dans le micro-canal 1 sans se toucher et sans toucher le périmètre du micro-canal 1 et qu'ils soient de préférence observables dans la chambre de réaction. L'invention concerne en outre un procédé destiné à effectuer un dosage multiplexé en se basant sur des micro-supports 2, caractérisé en ce qu'il améliore le transfert de masse, simplifie la préparation et l'exécution du dosage et facilite le relevé des réactions biologiques et de l'identité des micro-supports 2.

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