Production of human mutated proteins in human cells by means...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12N 15/90 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2297660

The invention concerns a process for the production of muteins of eukaryotic polypeptides in eukaryotic cells by means of homologous recombination. The invention additionally concerns a process for the production of human cells which are suitable for the production of human mutated proteins. Finally the invention concerns the human cells produced by the process and mutated human proteins obtainable therefrom as well as pharmaceutical preparations which contain these muteins.

L'invention concerne un procédé de production de protéines mutantes de polypeptides eucaryotes dans des cellules eucaryotes, par recombinaison homologue. L'invention concerne en outre un procédé de production de cellules humaines appropriées pour la production de protéines mutantes humaines. Enfin, l'invention concerne les cellules humaines fabriquées suivant ce procédé et les cellules mutantes ainsi obtenues, ainsi que des préparations pharmaceutiques renfermant ces protéines mutantes.

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