Hematology blood control and method for preparation of same

G - Physics – 01 – N

Patent

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Details

G01N 33/96 (2006.01) G01N 33/48 (2006.01) G01N 33/49 (2006.01)

Patent

CA 2407684

The control of the present invention involves a gentle chemical removal of red cells, leaving an intact white cell preparation. The preservation steps, namely the use of cross-linking agents as aldehydes, involve a step-wise process starting with very low concentrations of a cross-linking agent. The fixation part of the process outlined in this disclosure has also been applied to non-mammalian blood cells and therefor would appear to be a universal procedure for preparing all types of vertebrate blood cells.

Pour préparer la substance de test selon la présente invention on élimine chimiquement les érythrocytes de manière non agressive et on obtient ensuite une préparation de leucocytes à l'état intact. Les étapes de conservation consistant à utiliser des agents de réticulation tels que les aldéhydes, comprennent un processus progressif qui commence avec des concentrations très faibles d'un agent de réticulation. La partie de fixation du processus présentée dans cette invention a également été appliquée à des globules sanguins ne provenant pas de mammifères et par conséquent ce processus peut être considéré comme un procédé de préparation universel permettant de préparer tous les types de globules sanguins de vertébrés.

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