Preparation of biomolecules

B - Operations – Transporting – 01 – D

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B01D 11/04 (2006.01) C07K 1/20 (2006.01) G01N 33/537 (2006.01)

Patent

CA 2632328

The present invention relates to a method of separating a target from a liquid, which comprises providing a one polymer in an aqueous liquid, which polymer is comprises at least one hydrophobic portion; contacting the polymer- containing liquid with the liquid comprising the target; applying a stimulus to the resulting mixture; and maintaining it until a reversible phase separation is obtained, wherein one phase is polymer-rich and comprises target(s) and another phase is polymer-poor, and maintaining said stimulus, or applying a different stimulus and maintaining it until the polymer-rich phase has transformed into a substantially solid phase.

La présente invention concerne un procédé de séparation d'une cible d'un liquide, consistant à introduire un polymère dans un liquide aqueux, ledit polymère comprenant au moins une partie hydrophobe; à mettre en contact le liquide contenant le polymère avec le liquide contenant la cible; à appliquer un stimulus dans le mélange obtenu; et à le maintenir jusqu'à obtenir une séparation à phase réversible, une phase étant riche en polymère et comprenant une ou plusieurs cibles, et une autre phase étant pauvre en polymère; et à maintenir ledit stimulus, ou à appliquer un stimulus différent et à le maintenir jusqu'à ce que la phase riche en polymère se soit transformée en une phase sensiblement solide.

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