Production of ascorbic acid in plants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

IPC codes

C12N 15/53 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01) C12P 17/04 (2006.01)

Type

Patent

Patent number

CA 2288739

Description

The present invention relates to a polynucleotide in isolated form, which polynucleotide codes for a protein with the activity of the enzyme L-galactono- .gamma.-lactone dehydrogenase, which polynucleotide comprises at least the L- galactono-.gamma.-lactone dehydrogenase activity-determining parts of the coding part of the nucleotide sequence, which is shown in Fig. 3, or a sequence derived therefrom on the basis of the degeneration of the genetic code. The invention further relates to the use of the polynucleotide in the production of transgenic plants, plant cells or other eukaryotic cells.

La présente invention concerne un polynucléotide sous forme isolée, lequel code pour une protéine possédant l'activité d'une L-galactono-.gamma.-lactone déhydrogénase, et comprend au moins des parties, déterminant l'activité de la L-galactono-.gamma.-lactone déhydrogénase, de la partie codante de la séquence nucléotidique montrée sur la figure (3), ou une séquence dérivée de celle-ci d'après la dégénérescence du code génétique. L'invention concerne en outre l'utilisation de ce polynucléotide dans la production de plantes transgéniques, de cellules végétales ou d'autres cellules eucaryotes.

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