Inhibition of dna methyltransferase

C - Chemistry – Metallurgy – 12 – N

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C12N 9/00 (2006.01) A61K 31/70 (2006.01) A61K 39/395 (2006.01)

Patent

CA 2177031

The present invention relates to the interplay between the level of DNA methyltransferase and demethylase activities, to the role of the interplay between these levels on the proliferative differentiated, tumoriganic and homeostatic state of the cell, and to the DNA methyltransferase and demethylase as therapeutic targets. The invention further relates to a redution of the level of DNA methylation through inhibitors and antagonist in ordar to inhibit the excessive activity or hypermethylation of DNA MeTase in cancer cells to induce the original cellular tumor suppressing program, to turn on alternative gene expression programs, to provide therapeutics directed at a nodal point of regulation of genetic information, and to modulate the genral level of methylase and demethylase enzymatic activity of a cell to permit specific changes in the methylation pattern of a cell.

L'invention concerne l'interaction entre le niveau des activités méthyltransférase et déméthylase de l'ADN, le rôle de l'interaction existant entre ces niveaux sur l'état prolifératif, différencié, tumorigène et homéostatique de la cellule, ainsi que la méthyltransférase et la déméthylase de l'ADN en guise de cibles thérapeutiques. L'invention concerne en outre une réduction du niveau de méthylation de l'ADN au moyen d'inhibiteurs et d'un antagoniste pour inhiber l'activité excessive ou l'hyperméthylation de la MeTase de l'ADN dans des cellules cancéreuses, pour induire le programme cellulaire originel de suppression de tumeurs, pour enclencher des programmes d'expression génique de rechange, pour offrir de thérapies orientées vers le point nodal de la régulation de l'information génétique, et pour moduler le niveau général d'activité enzymatique de la méthylase et de la déméthylase d'une cellule afin de permettre des changements spécifiques dans les modalités de méthylation de cette cellule.

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