Resistance circuit stabilization and pulse duration control...

C - Chemistry – Metallurgy – 12 – M

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C12M 1/42 (2006.01) C12N 13/00 (2006.01) C12N 15/87 (2006.01) G01R 27/02 (2006.01)

Patent

CA 2468755

Systems, methods and apparatus for stabilizing resistance measurements in electroporation systems so as to achieve high-accuracy measurements, and for controlling the pulse duration, or time constant, of electroporation pulses. Circuitry is provided to accurately determine the resistance Rs of a sample 25 in the electroporation system, for example a sample 25 provided in an electroporation cuvette. Additionally, a resistance control system is provided to automatically measure the resistance of a sample 25, determine a capacitance and determine a parallel add-on resistance R2 that substantially provides a desired pulse duration (time constant) for an electroporation pulse.

Systèmes, procédés et appareil permettant de stabiliser des mesures de résistance dans des systèmes d'électroporation dans le but d'obtenir de relevés de grande précision et de commander la durée (ou constante de temps) d'impulsions d'électroporation. Des circuits permettent de déterminer avec précision la résistance d'un échantillon dans un système d'électroporation, tel qu'un échantillon disposé dans une cuvette d'électroporation. Par ailleurs, un système de commande de résistance mesure automatiquement la résistance d'un échantillon, détermine une capacitance et une résistance supplémentaire parallèle qui garantit sensiblement la durée requise (constante de temps) pour une impulsion d'électroporation.

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