Disposable laboratory articles for analysis and diagnosis

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2673929

The invention relates to disposable articles for a laboratory and in particular to prepared reaction vessels for conducting the polymerase chain reaction for analytical and diagnostic purposes. The invention further relates to a method for the stable storage and drying of minute amounts of oligonucleotides and reference nucleic acids in reaction vessels. The oligonucleotides and the nucleic acids are dried on the wall of the reaction vessel in the presence of 1 to 5 mM trehalose, without any further components.

L'invention concerne des articles de laboratoire à usage unique et notamment des récipients de réaction préparés pour effectuer la réaction de polymérisation en chaîne (PCR) à des fins d'analyse et de diagnostic. Elle concerne aussi un procédé permettant de stocker à l'état sec et stable des quantités infimes d'oligonucléotides et d'acides nucléiques de référence dans des récipients de réaction. Ce procédé est caractérisé en ce que les oligonucléotides et les acides nucléiques sont - en l'absence de tout autre composant - séchés sur la paroi du récipient de réaction en présence de 1 à 5 mM de tréhalose.

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