Diagnostic methods for protein profiling

Details Diagnostic methods for protein profiling Diagnostic methods for protein profiling

G - Physics

01

N

G01N 33/68 (2006.01) C07K 14/435 (2006.01) C07K 16/00 (2006.01) C12N 15/09 (2006.01) G01N 27/27 (2006.01) G01N 33/00 (2006.01) G01N 33/15 (2006.01) G01N 33/483 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 37/00 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2469492

Methods for establishing a protein expression profile of a biological sample, by contacting a biological sample with a ubiquitin-binding protein such that ubiquitinated and specific non-ubiquitinated protein (the IPS) bind to the ubiquitin-binding protein; isolating the IPS proteins; and analyzing the isolated IPS proteins, wherein an expression profile is generated.

La présente invention concerne des procédés permettant de dresser le profil d'expression d'une protéine d'un échantillon biologique. A cet effet, on prend un échantillon biologique, et on le met en contact avec une protéine se liant à l'ubiquitine de façon que la protéine ubiquitinée et non ubiquitinée spécifique (l'IPS) se lie à la protéine se liant à l'ubiquitine. Ces procédés permettent également d'une part d'isoler les protéines IPS, et d'autre part d'analyser les protéines IPS isolées, avec génération d'un profil d'expression.

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