Cassette containing growth medium

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/04 (2006.01)

Patent

CA 2623408

The invention features devices for capturing and culturing cells (e.g., microorganisms, cells containing microorganisms, or cells from eukaryotic cell cultures) and methods of using these devices. One device is a cassette containing growth media that may be employed in an automated rapid enumeration system. The cassette has, for example, been enhanced with features for controlling surface flatness, optical imaging, controlled dehydration of semi solid nutrient media, controlled air and particle exchange, and automated handling. Another device of the invention is a filtration funnel that may used to concentrate cells in a sample onto a membrane.

La présente invention se rapporte à des dispositifs de capture et de culture de cellules (par ex., de micro-organismes, de cellules contenant des micro-organismes, ou de cellules issues de cultures de cellules eucaryotes), et à des procédés d'utilisation desdits dispositifs. Un dispositif selon l'invention se présente sous la forme d'une cassette contenant un milieu de croissance, qui peut être utilisée dans un système de dénombrement rapide automatisé. Ladite cassette a par exemple été perfectionnée par des éléments de régulation de la planéité de surface, d'imagerie optique, de déshydratation contrôlée de milieux nutritifs semi-solides, d'échange contrôlé d'air et de particules, et de manipulation automatisée. Un autre dispositif selon l'invention se présente sous la forme d'un entonnoir de filtration, qui peut servir à concentrer des cellules contenues dans un échantillon sur une membrane.

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