C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
C07H 1/00 (2006.01) C12P 19/12 (2006.01)
Patent
CA 2623234
A process is described which uses whole cell preparations, immobilized or without immobilization, of a microorganism, includingAureobasidium pullulans, capable of forming enzymes of the group of fructosyltransferases, for catalyzing a reaction between sucrose and 6-0-protected glucose for formation of 6-0-protected sucrose, an intermediate in synthesis of trichlorogaiactosucrose. 6-0-protected Sucrose is separated from high molecular weight by-products of the reaction having molecular weight of 500 daltons and more by reverse osmosis, and further purified by column chromatography.
L'invention concerne un procédé qui utilise des préparations de cellules complètes, immobilisées ou non immobilisées, d'un micro-organisme, notamment Aureobasidium pullulans, capable de former des enzymes du groupe des fructosyltransférases, afin de catalyser une réaction entre le saccharose et le glucose 6-0-protégé pour la formation de saccharose 6-0-protégé, un intermédiaire dans la synthèse du trichlorogalactosaccharose. Le saccharose 6-0-protégé est séparé par osmose inverse des sous-produits à poids moléculaire élevé de la réaction dont le poids moléculaire est égal ou supérieur à 500 daltons puis purifié par chromatographie sur colonne.
Aurora Sundeep
Ratnam Rakesh
Subramaniyam P.
Pharmed Medicare Pvt. Ltd.
Ridout & Maybee Llp
V. B. Medicare Private Limited
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