Method and kit for evaluating risk of ovarian cancer in...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2198801

The association between the presence of one or more rare (infrequent) alleles of the HRAS variable tandem repeat (VTR) polymorphism and the incidence of ovarian cancer in women who harbor a BRCAl mutation can be used for evaluating risk of ovarian cancer in a human patient. The patient is tested for the presence of a mutation in the BRCAl gene; and to determine the polymorphic form of the HRASl variable tandem repeat region. The presence of both a mutation in the BRCAl gene and a rare polymorphic form of the HRASl variable tandem repeat region is indicative of an elevated risk of developing ovarian cancer. A kit for performing this evaluation includes reagents necessary for performing a test for the BRCAl mutation and to evaluate the polymorphic form of the HRASl variable tandem repeat region.

L'association entre la présence d'un allèle rare (peu fréquent) ou plus du polymorphisme du minisatellite HRAS et l'incidence du cancer des ovaires chez les femmes porteuses d'une mutation BRCAl peut être utilisée pour évaluer le risque de cancer des ovaires chez les humains. On vérifie la présence d'une mutation dans le gène BRCAl chez la femme, puis on détermine le polymorphisme dans la région du minisatellite HRASl. La présence de la mutation du gène BRCAl et d'un polymorphisme rare dans la région du minisatellite HRASl est indicative d'un risque accru de cancer des ovaires. Une trousse pour effectuer cette évaluation comprend les réactifs nécessaires pour réaliser un test visant à détecter la mutation BRCAl et pour évaluer le polymorphisme de la région du minisatellite HRASl.

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