Method for purifying a protein of the cystine-knot superfamily

Details Method for purifying a protein of the cystine-knot superfamily Method for purifying a protein of the cystine-knot superfamily

C - Chemistry, Metallurgy

07

K

C07K 1/10 (2006.01) C07K 14/705 (2006.01)

Patent

CA 2572947

The present invention relates to a two-step method for isolating proteins from the cystine-knot superfamily based on dye ligand affinity chromatography and reversed-phase chromatography. Advantageously, the method can be performed in a relatively short period of time, involves inexpensive reagents, and requires little sample preparation before and during the purification process. Protein fusions between cystine-knot proteins and proteins of interest are further provided for the isolation of said protein of interest or complexes containing said protein of interest using the two-step method disclosed.

La présente invention concerne un procédé en deux étapes servant à isoler des protéines appartenant à la superfamille des protéines à noeud cystine et basé sur la chromatographie d'affinité avec un ligand colorant et la chromatographie en phase inverse. De façon avantageuse, le procédé peut être réalisé sur une durée relativement courte, met en jeu des réactifs bon marché et nécessite peu de préparation de l'échantillon avant et pendant le processus de purification. L'invention concerne également des fusions de protéines entre des protéines à noeud cystine et des protéines présentant un intérêt pour isoler ladite protéine présentant un intérêt ou des complexes contenant ladite protéine présentant un intérêt en utilisant le procédé en deux étapes exposé.

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