Groel muteins with improved stability

Details Groel muteins with improved stability Groel muteins with improved stability

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) A61K 38/16 (2006.01) C07K 14/245 (2006.01) C07K 16/12 (2006.01) C12N 15/62 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2375095

A GroEL chaperone polypeptide, or homologue thereof, or fragment thereof having protein refolding activity, comprising one or more amino acid modifications at any one of amino acid residues (207, 212, 217, 223, 233, 267, 271, 294, 305, 308 and 326) of the GroEL amino acid sequence shown as SEQ I.D. No. 1 or their equivalent positions in other homologous chaperone polypeptides is provided.

L'invention concerne un polypeptide chaperon GroEL ou un homologue de celui-ci ou un fragment de celui-ci possédant une activité de repliement de protéine, qui comprend une ou plusieurs modifications d'acides aminés en tout reste des acides aminés 207, 212, 217, 223, 267, 271, 294, 305, 308 et 326 de la séquence d'acides aminés GroEL représentée par SEQ I.D. No. 1 ou au niveau de leurs positions équivalentes dans d'autres polypeptides chaperons homologues.

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