Spectroscopic method for the detection of analytes

G - Physics – 01 – N

Patent

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Details

G01N 33/58 (2006.01) G01N 33/533 (2006.01) G01N 33/542 (2006.01)

Patent

CA 2605809

The invention relates to a method for detecting one or several analytes, in particular pathogens, viruses, prions, bacteria, parasites, pharmaceutical agents, antibiotics, cytostatic agents, psychopharmaceuticals, narcotics, pain killers, heart medicaments, metabolites, coagulation inhibitors, hormones, interleucines and cytokines, doping agents, drugs, toxins, poisons, pesticides, insecticides, wood protecting agents, herbicides, fungicides, explosives, vitamins and aromas, wherein a conjugate of the analytes is provided with an europium-cryptate-fluorophor and/or a terbium-cryptate- fluorophor together with an anti-body which is specific to the analytes and an anti-body which is specific to the europium-cryptate-fluorophor and/or the terbium-cryptate-fluorophor and is determined by adding the fluorescent solution in a spectropic manner to the analytes. The invention also relates to conjugates from the analytes and the europium-cryptate-fluorophor and/or terbium-cyptate-fluorophor and a kit for detecting analytes.

La présente invention concerne des procédés de détection d'un ou plusieurs analytes, notamment de pathogènes, virus, prions, bactéries, parasites, agents pharmaceutiques, antibiotiques, cytostatiques, agents psychopharmaceutiques, narcotiques, analgésiques, médicaments pour le coeur, métabolites, inhibiteurs de coagulation, hormones, interleukines, cytokines, dopants, drogues, toxines, poisons, pesticides, insecticides, xyloprotecteurs, herbicides, fongicides, explosifs, vitamines et arômes, par mise en oeuvre d'un conjugué de l'analyte, d'un europium-kryptate-fluorophore ou d'un terbium-kryptate-fluorophore et d'un anticorps spécifique à l'analyte et d'un anticorps spécifique à l'europium-kryptate-fluorophore ou au terbium-kryptate-fluorophore, et détermination spectroscopique de l'extinction de fluorescence lors de l'addition de l'analyte. L'invention concerne également des conjugués de l'analyte, de l'europium-kryptate-fluorophore et du terbium-kryptate-fluorophore, ainsi qu'un ensemble de détection d'analytes.

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