Cell separation composition, kit and method

A - Human Necessities – 61 – L

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Details

A61L 2/02 (2006.01) A61L 2/08 (2006.01) B01J 20/32 (2006.01) B01L 3/14 (2006.01) G01N 33/49 (2006.01) G01N 33/554 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2239729

Disclosed are a kit, composition and method for cell separation. The kit includes a centrifugable container and an organosilanized silica particle-based cell separation suspension suitable for density gradient separation, containing a polylactam and sterilized by treatment with ionizing radiation. The composition includes a silanized silica particle-based suspension for cell separation which contains at least 0.05 % of a polylactam, and preferably treated by ionizing radiation. Also disclosed is a method of isolating rare blood cells from a blood cell mixture.

L'invention concerne une composition, une trousse et un procédé permettant de séparer des cellules. La trousse comprend un récipient centrifugeur et une suspension pour séparation de cellules à base de particules de silice organosilanisée, convenant pour la séparation par gradient de densité, contenant un polylactame et stérilisée par traitement par radiations ionisantes. La composition comprend une suspension à base de silice silanisée convenant pour la séparation de cellules, qui contient au moins 0,05 % d'un polylactame et qui est de préférence traitée par radiations ionisantes. L'invention concerne également un procédé permettant d'isoler des cellules sanguines rares dans un mélange de cellules sanguines.

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