A - Human Necessities – 61 – L
Patent
A - Human Necessities
61
L
A61L 2/02 (2006.01) A61L 2/08 (2006.01) B01J 20/32 (2006.01) B01L 3/14 (2006.01) G01N 33/49 (2006.01) G01N 33/554 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2239729
Disclosed are a kit, composition and method for cell separation. The kit includes a centrifugable container and an organosilanized silica particle-based cell separation suspension suitable for density gradient separation, containing a polylactam and sterilized by treatment with ionizing radiation. The composition includes a silanized silica particle-based suspension for cell separation which contains at least 0.05 % of a polylactam, and preferably treated by ionizing radiation. Also disclosed is a method of isolating rare blood cells from a blood cell mixture.
L'invention concerne une composition, une trousse et un procédé permettant de séparer des cellules. La trousse comprend un récipient centrifugeur et une suspension pour séparation de cellules à base de particules de silice organosilanisée, convenant pour la séparation par gradient de densité, contenant un polylactame et stérilisée par traitement par radiations ionisantes. La composition comprend une suspension à base de silice silanisée convenant pour la séparation de cellules, qui contient au moins 0,05 % d'un polylactame et qui est de préférence traitée par radiations ionisantes. L'invention concerne également un procédé permettant d'isoler des cellules sanguines rares dans un mélange de cellules sanguines.
Palathumpat Varghese
Van Vlasselaer Peter
Dendreon Corporation
Gowling Lafleur Henderson Llp
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