Method for detecting intimin producing microorganisms

Details Method for detecting intimin producing microorganisms Method for detecting intimin producing microorganisms

G - Physics

01

N

G01N 33/569 (2006.01) C07K 14/24 (2006.01) C07K 14/245 (2006.01) C07K 16/12 (2006.01) C12Q 1/04 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2320753

Methods of detecting and/or diagnosing intimin-expressing microorganisms such as enteropathogenic Escherichia coli (EPEC), enterohemorrhagic E. coli, C. rodentium and/or RDEC-1, as well as kits for use in such methods are provided. In particular, methods based antisera raised against conserved regions are described, as well as polypeptide regions useful in the production of antisera. Vaccines based on such peptides are also described herein. In addition, methods of typing/classification of such bacteria are also described herein. Finally, methods for the isolation of intimin-expressing microorganisms are also provided.

La présente invention concerne des procédés permettant de détecter et/ou de diagnostiquer la présence de micro-organismes exprimant l'intimine, tels que l'Escherichia coli entérophatogène, l'Escherichia coli entérohémorragique, le C. rodentium et/ou le RDEC-1, ainsi que des trousses à utiliser avec ces procédés. L'invention se rapporte en particulier à des antisérums dirigés contre des régions conservées obtenus selon les procédés de l'invention, et à des régions polypeptidiques utilisées dans la production des antisérums. L'invention concerne également des vaccins basés sur ces peptides, ainsi que des procédés de typage/classification des bactéries précitées. L'invention se rapporte enfin à des procédés permettant d'isoler les micro-organismes exprimant l'intimine.

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