Cost effective media for large scale insect cell culture

C - Chemistry – Metallurgy – 12 – N

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C12N 5/02 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2413512

A serum-free insect cell culture medium is described which provides improvements in the maximum cell density supported and replication of insect viruses within these cells, at a significantly lower cost than commercially- available media. An improved method for preparing a lipid emulsion which is easy to scale and which produces a more stable emulsion with a longer shelf life is also described.

L'invention concerne un milieu de culture de cellules d'insectes sans s~rum permettant d'obtenir des am~liorations de la densit~ cellulaire maximum support~e et de r~pliquer des virus d'insectes dans ces cellules, ~ un coÛt nettement inf~rieur ~ celui des milieux disponibles sur le march~. L'invention concerne ~galement un proc~d~ am~lior~ de pr~paration d'une ~mulsion grasse facile ~ graduer et qui produit une ~mulsion plus stable ~ dur~e de conservation plus longue.

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